Extracting and counting normal cells are simple tasks if matlab detected cells are typical cells and include small variety of overlapping cells with regular shape. For this variety of easy case, matlab idea of employing Hough Transform can also be very helpful as a result of matlab can produce engineering good performance. With identical idea to , Venkatalakshmi and Thilagavathi have also applied round Hough Transform approach to count matlab RBCs from microscopic photos after performing preprocessing steps reminiscent of HSV transformation, S channel extraction, histogram thresholding morphological operations, XOR logical operation, and Canny edge detection. However, once again, this proposed idea is less tolerant to any overlapping cells or abnormal cells’ structure. Later, Maitra et al. provided engineering composition method to extract red cell from five microscopic pictures; those steps comprise spatial smoothing and filtering, adaptive histogram equalization, and edge detection.